Ikhithi Yokuhlola Esheshayo Ye-Syphilis (Lateral Chromatography)

Imininingwane Yomkhiqizo:

Ukusetshenziswa okuhlosiwe:

Ikhithi Yokuhlola Esheshayo Ye-Syphilis (Lateral Chromatography) ukuhlolwa kwe-chromatographic immunoassay ukuze kutholwe ikhwalithi yamasosha omzimba e-TP egazini lonke, i-serum noma i-plasma ukusiza ekuxilongeni ugcunsula.

Izimiso Zokuhlola:

Ikhithi Yokuhlola Okusheshayo Ye-Syphilis isuselwe ekuhlolweni kwe-immunochromatographic ukuze kutholwe amasosha omzimba e-TP egazini lonke, i-serum noma i-plasma.Ngesikhathi sokuhlolwa, amasosha omzimba e-TP ahlangana nama-antigen e-TP abhalwe ezinhlayiyeni eziyindilinga ezinombala ukuze akhe inkimbinkimbi yamasosha omzimba.Ngenxa yesenzo se-capillary, i-immune complex complex igeleza kulwelwesi.Uma isampula iqukethe amasosha omzimba e-TP, izothathwa indawo yokuhlola ehlanganiswe ngaphambili futhi yakhe umugqa wokuhlola obonakalayo.Ukuze usebenze njengokulawula inqubo, kuzovela ulayini wokulawula onemibala uma ukuhlolwa kwenziwe ngendlela efanele

Okuqukethwe Okuyinhloko:

Okwe-Stripe:

Okwekhasethi:

Ukugeleza Kokusebenza

• Isinyathelo 1: Ukulungiselela Isampula

Ikhithi Yokuhlola Esheshayo Ye-Syphilis (Lateral Chromatography) ingenziwa kusetshenziswa igazi eliphelele, i-serum noma i-plasma.

1. Hlukanisa i-serum noma i-plasma egazini ngokushesha ngangokunokwenzeka ukuze ugweme i-hemolysis.Sebenzisa kuphela izibonelo ezicacile ezingenayo i-hemolyzed.

2. Ukuhlolwa kufanele kwenziwe ngokushesha ngemva kokuba izifanekiso seziqoqwe.Uma ukuhlola kungeke kuqedwe ngokushesha, isampula ye-serum ne-plasma kufanele igcinwe ku-2-8°C kuze kube yizinsuku ezi-3, ukuze igcinwe isikhathi eside, izibonelo kufanele zigcinwe ku -20℃.Igazi eliphelele eliqoqwe nge-venipuncture kufanele ligcinwe ku-2-8°C uma ukuhlolwa kuzokwenziwa phakathi nezinsuku ezi-2 zokuqoqwa.Ungawaqandi amasampula egazi eliphelele.Igazi eliphelele eliqoqwe ngothi lweminwe kufanele lihlolwe ngokushesha.

3. Amasampula kufanele abuyiselwe ekamelweni lokushisa ngaphambi kokuhlolwa.Amasampula aqandisiwe adingeka ukuthi ancibilikiswe ngokuphelele futhi axutshwe kahle ngaphambi kokuhlolwa, ukugwema ukuqanda nokuncibilika ngokuphindaphindiwe.

4. Uma izifanekiso zizothunyelwa, kufanele zipakishwe ngokuhambisana nemithetho yendawo ehlanganisa ukuthuthwa kwama-etiologic agents.

• Isinyathelo sesi-2: Ukuhlola

Vumela umugqa wokuhlola/ikhasethi, isifanekiso, isampula lokuhlanjululwa ukuze kufinyelele igumbi

izinga lokushisa (15-30 ° C) ngaphambi kokuhlolwa.

1. Khipha umucu wokuhlola/ikhasethi esikhwameni esivaliwe bese ulisebenzisa phakathi nemizuzu engama-30.

2. Beka i-test strip/cassette endaweni ehlanzekile nesezingeni.

2.1 Ngezifanekiso zeSerum noma zePlasma:

Bamba i-dropper ngokuqondile, udwebe isifanekiso phezulu koLayini Wokugcwalisa ongezansi (cishe u-40uL), bese udlulisela isifanekiso kumthombo wesampula (S) we-test strip/cassette, bese wengeza iconsi elingu-1 lesampula le-diluent (cishe 40uL) bese uqala. isibali sikhathi.Gwema ukuvalela amabhamuza omoya endaweni yesifanekiso(S).Bheka umfanekiso ngezansi.

2.2 Izifanekiso Zegazi Eliphelele (I-Venipuncture/Fingerstick):

Bamba i-dropper ngokuqondile, dweba isifanekiso kulayini Wokugcwalisa ongaphezulu (cishe u-80uL), bese udlulisela igazi eliphelele esibonelweni somthombo (S) we-test strip/cassette, bese wengeza iconsi elingu-1 lesampula le-diluent (cishe 40uL) bese uqala isibali sikhathi.Gwema ukuvalela amabhamuza omoya endaweni yesifanekiso(S).Bheka umfanekiso ngezansi.

• Isinyathelo sesi-3: Ukufunda

3. Funda umphumela ngemuva kwemizuzu eyi-10-20.Umphumela awuvumelekile ngemva kwemizuzu engu-20.

Ukutolika imiphumela

1. Umphumela Omuhle

Uma kokubili ulayini wokulawula ikhwalithi u-C kanye nolayini we-T wokuhlonza kuvela, kubonisa ukuthi isifanekiso siqukethe inani elibonakalayo lamasosha omzimba e-TP, futhi umphumela uba positive kugcunsula.

2. Umphumela Ongemuhle

Uma kuphela ulayini u-C wokulawula ikhwalithi uvela futhi ulayini we-T wokutholwa ungabonisi umbala, kubonisa ukuthi amasosha omzimba e-TP awabonakali kusifanekiso.futhi umphumela uba negative kugcunsula.

3. Umphumela Ongavumelekile

Alikho ibhande elinemibala elibonakalayo elivela kulayini wokulawula ngemva kokwenza uhlolo, umphumela wokuhlola awuvumelekile.Phinda uhlole isampula.

Ulwazi Lwe-oda: