I-Insect Cell Protein Expression
Uhlelo lwe-insect cell expression luwuhlelo oluvame ukusetshenziswa lwe-eukaryotic lokubonisa amaprotheni amangqamuzana amakhulu.Uma kuqhathaniswa namaseli ezilwane ezincelisayo, izimo zesiko lamaseli ezinambuzane zilula futhi azidingi i-CO2.I-Baculovirus uhlobo lwegciwane le-DNA elinemicu ephindwe kabili elinamaseli ezinambuzane njengomsingathi wemvelo.Inezinhlobo ezithile eziqondile, ayitheleli izilwane ezinomgogodla, futhi ayinangozi kubantu nasemfuyweni.I-sf9, esetshenziswa kakhulu njengeseli yokusingatha, ivela ku-planktonic noma ihambisana nesiko.I-sf9 ifaneleka kakhulu ekukhulumeni ngezinga elikhulu, futhi ingasetshenziselwa ukucubungula nokuguqulwa okulandelayo kwamaprotheni afana ne-phosphorylation, i-glycosylation, ne-acylation.Uhlelo lwe-insect cell expression lungasetshenziswa futhi ukuveza izakhi zofuzo eziningi, futhi lungaveza amaprotheni anobuthi njengama-peptide e-antimicrobial.
| Izinto Zesevisi | Isikhathi Sokuhola (BD) |
| Ukwenza ngcono kwe-Codon, ukuhlanganiswa kofuzo kanye ne-subcloning | 5-10 |
| I-P1 generation virus incubation kanye nenkulumo yesikali esincane | 10-15 |
| I-P2 generation virus incubation, ukuvezwa okukhulu nokuhlanzwa, ukulethwa kwamaprotheni ahlanziwe kanye nombiko wokuhlola |