I-Prokaryotic Protein Expression
Uhlelo lwe-prokaryotic E. coli expression luvunywa kabanzi njengohlelo olungabizi kakhulu, oluvuthwe ngokobuchwepheshe, futhi olusetshenziswa kakhulu ekuvezeni amaprotheni.Kwa-Bioantibody, sizibophezele ekunikezeni amakhasimende ethu izinsiza eziphelele, eziphuma endaweni eyodwa kusukela ekuhlanganiseni izakhi zofuzo kuya ekuvezeni amaprotheni kanye nokuhlanzwa.Amasevisi ethu ahlanganisa ukwenziwa ngcono kwe-codon kwamahhala kanye nokusetshenziswa kobuchwepheshe bethu bobunikazi ukuze kubhekwane nanoma yiziphi izinkinga ezihlobene nokusho okuphansi nokunganyibiliki okungase kuvele phakathi nayo yonke inqubo yokuvezwa nokuhlanza.Amakhasimende ethu adinga kuphela ukuhlinzeka ngofuzo noma ukulandelana kwe-amino acid yephrotheni futhi singaletha amaprotheni ekhwalithi ephezulu ngokushesha nje emavikini amathathu.Ukwengeza, i-Bioantibody inikeza izinsiza zokususwa kwe-endotoxin kanye nokumaka eziklanyelwe izidingo ezithile zamakhasimende ethu.Sizibophezele ekuletheni imiphumela futhi sithembisa ukuthi ngeke sikukhokhise noma yiziphi izindleko uma iphrotheni yokugcina ingavezwanga.
| Izinto Zesevisi | Okuqukethwe Kokuhlola | Isikhathi Sokuhola (BD) |
| I-Gene Synthesis | Ukwenza ngcono kwe-Codon, ukuhlanganiswa kofuzo kanye ne-subcloning. | 5-10 |
| Ukukhonjwa Kwenkulumo Nokuhlaziywa Kokuncibilika | 1. Ukuguqulwa kanye ne-incubation, ukutholwa kwesisho nge-SDS-PAGE.2. Ukuhlaziywa kokuncibilika, i-SDS-PAGE kanye nokutholwa kwe-WB | 10 |
| Ukufukamela okukhulu nokuhlanza, amaprotheni wokugcina (ubumsulwa> 85%, 90%, 95%) kanye nombiko wokuhlola ojwayelekile | Ukuhlanzwa kobudlelwane (ikholomu ye-Ni, i-MBP, i-GST) |
Uma isakhi sofuzo sihlanganisweI-bioantibody, i-plasmid eyakhiwe izofakwa kokulethwayo.