I-Recombinant Monkeypox Virus A29L Protein, C-Ithegi yakhe

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Umthombo	I-Monkeypox Virus(strain Zaire-96-I-16)
Expression Host	E.coli
Maka	C-Ithegi yakhe
Isicelo	Ilungele ukusetshenziswa kuma-immunoassays. Ilabhorethri ngayinye kufanele inqume i-titer yokusebenza efanele ukuze isetshenziswe ezinhlelweni zayo ikakhulukazi.
Ulwazi oluvamile	I-recombinant monkeypox virus A29L protein ikhiqizwa i-E.coli expression system kanye ne- umbhalo wekhodi wofuzo lwe-Met1-Glu110 uvezwa nge-His-tag ku-C-terminus.

Ubumsulwa	>95% njengoba kunqunywa i-SDS-PAGE.
I-Molecular Mass	I-recombinant monkeypox virus A29L protein equkethe ama-amino acid ayi-125 futhi inesisindo samangqamuzana esibaliwe esingu-14.2 kDa.
Isilondolozi somkhiqizo	20 mM Tris, 300 mM NaCl, pH 9.0.
Isitoreji	Igcine ngaphansi kwezimo eziyinyumba ku -20℃ ukuya ku -80 ℃ uma yamukelwa. Ncoma ukuthi i-aliquot iphrotheni ibe ngamanani amancane ukuze uthole isitoreji esiphezulu.