I-Mammalian Cell Protein Expression

Isistimu yeseli yezilwane ezincelisayo isebenzisa amaseli ezilwane ezincelisayo njenge-HEK293 ne-CHO futhi inika amandla ukuguqulwa kwangemuva kokuhumusha, okuhlanganisa ukugoqa kanye ne-glycosylation eyinkimbinkimbi, okuholela emaphrotheni afana kakhulu nozakwabo bemvelo ngokomsebenzi.Njengomphumela wale nzuzo ehlukile, uhlelo lokuvezwa kwamangqamuzana ezincelisayo ludlala indima ebalulekile ekutholweni kwezakhi zofuzo, ukwakheka kwamaprotheni nocwaningo lomsebenzi, kanye nokuthuthukiswa kwezidakamizwa zobunjiniyela bezakhi zofuzo.Kodwa-ke, uhlelo lwamanje lokuvezwa kweseli yezilwane ezincelisayo linemikhawulo ethile, njengesikhathi eside sokushintsha kanye nezindleko zokukhiqiza eziphezulu.

Inqubo Yesevisi

Izinto Zesevisi

Izinto Zesevisi	Isikhathi Sokuhola (BD)
I-20mL ye-high-density expression kanye nesevisi yokuhlanza	20-25
I-1-10L ye-high density expression kanye nenkonzo yokuhlanza	20-25

Izinzuzo Zesevisi

Ukusebenzisa i-HEK293 noma i-CHO njengeseli yokusingatha.I-serum-free incubation, i-High-density Expression, I-High-Density Efficiency, I-High Expression Level, Futhi Akukho Ukuphazanyiswa Kwangaphandle.

Hlangana nezidingo ezingokwezifiso zamakhasimende zokuhlanzeka kwamaprotheni, ukugxilisa ingqondo, i-endotoxin, i-Buffer, njll.

Indlela yoku-oda

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