Ukusetshenziswa Okuhlosiwe
Ikhithi yokuhlonza i-malaria antigen yakhelwe njengendlela elula, esheshayo, esezingeni eliphezulu futhi engabizi kakhulu ukuze kutholwe kanyekanye kanye nokuhlukanisa i-Plasmodium falciparum (Pf) ne-Plasmodium vivax (Pv) egazini lomuntu lonke noma ngomunwe.Le divayisi ihloselwe ukusetshenziswa njengokuhlolwa kokuhlola futhi isetshenziselwe ukuxilonga okuyisizayo kokutheleleka kwe-P. f ne-Pv.
Isimiso Sokuhlola
Ikhithi yokuhlola i-malaria antigen (Lateral chromatography) isekelwe kumgomo we-microsphere double antibody sandwich indlela yokunquma ngokushesha ikhwalithi ye-Pf/Pv antigen egazini lomuntu lonke noma igazi eliphelele ngomunwe.I-microsphere imakwe nge-anti-HRP-2 antibody (specific to Pf) ebhendini ye-T1 kanye ne-anti-PLDH antibody(specific to Pv) ebhendini ye-T2, futhi i-anti-mouse IgG polyclonal antibody imbozwe endaweni yokulawula ikhwalithi (C. ).Uma isampula iqukethe i-malaria HRP2 noma i-pLDH antigen futhi ukugxila kungaphezulu komkhawulo omncane wokutholwa, ovunyelwe ukuthi usabele nge-colloidal microsphere ehuqwe nge-Mal-antibody ukuze yakhe inkimbinkimbi ye-antibody-antigen.I-complex ibe isihamba ilandelana kulwelwesi futhi ngokulandelana ibophezela ku-antibody enganyakazi kulwelwesi olukhiqiza umugqa ophinki endaweni yokuhlola, okubonisa umphumela omuhle.Ukuba khona kolayini wokulawula kubonisa ukuthi ukuhlolwa kwenziwe ngendlela efanele kungakhathalekile ukuba khona kwe-Pf/Pv antigen.
IngxenyeREF | B013C-01 | B013C-25 |
Hlola Ikhasethi | 1 ukuhlola | 25 izivivinyo |
I-Diluent yesampula | 1 ibhodlela | 1 ibhodlela |
I-Dropper | 1 isiqephu | 25 ama-PC |
Imiyalo Yokusetshenziswa | 1 isiqephu | 1 isiqephu |
Isitifiketi Sokuhambisana | 1 isiqephu | 1 isiqephu |
Qoqa igazi eliphelele lomuntu noma umunwe wakho ngendlela efanele.
1. Khipha ishubhu lokukhipha ekhithi kanye nebhokisi lokuhlola esikhwameni sefilimu ngokuklebhula inotshi.Ibeke endizeni evundlile.
2. Vula isikhwama se-aluminium foil sekhadi lokuhlola.Susa ikhadi lokuhlola bese ulibeka livundlile etafuleni.
3. Engeza isixazululo sesampula se-dilution esingu-60μL ngokushesha.Qala ukubala.
Ngemuva kwemizuzu engu-20, funda imiphumela ngokubuka.(Qaphela: UNGAYIFUNDI imiphumela ngemva kwemizuzu engama-30!)
1.Pf Okuhle
Ukuba khona kwamabhendi amabili anombala ("T1" kanye "C") phakathi kwewindi lemiphumela kubonisa ukuthi i-Pf Positive.
2.Pv Positive
Ukuba khona kwamabhendi amabili anemibala ("T2" kanye "C") ngaphakathi kwewindi lomphumela kubonisa i-Pv
3.Okuhle.I-Pf ne-Pv Positive
Ukuba khona kwamabhendi anemibala emithathu ("T1", T2"kanye "C") ngaphakathi kwewindi lomphumela kungase kubonise ukutheleleka okuxubile kwe-P. f ne-Pan.
4.Umphumela Ongemuhle
Ukuba khona komugqa wokulawula kuphela(C) ngaphakathi kwewindi lomphumela kubonisa umphumela ongemuhle.
5.Umphumela Ongavumelekile
Uma lingekho ibhendi elivela endaweni yokulawula(C), imiphumela yokuhlolwa ayivumelekile kungakhathaliseki ubukhona noma ukungabikho komugqa endaweni yokuhlola(T).Isikhombisi-ndlela kungenzeka asilandelwanga ngendlela efanele noma kungenzeka ukuthi ukuhlola konakele Kunconywa ukuthi uphinde ukuhlola usebenzisa idivayisi entsha.
Igama Lomkhiqizo | Ikati.Cha | Usayizi | Isifanekiso | I-Shelf Life | Trans.& Sto.Temp. |
Umalaleveva HRP2/pLDH (Pf/Pv) Ikhithi Yokuhlola Esheshayo Ye-Antigen (I-Lateral chromatography) | B013C-01 | 1 test/kit | Igazi Eliphelele/Umunwe Womunwe | Izinyanga ezingu-18 | 2-30℃ / 36-86℉ |
B013C-25 | Izivivinyo ezingama-25/ikhithi |