Ikhithi Yokuhlola Okusheshayo Ye-Monkeypox Virus Antigen (i-Lateral Chromatography)
Imininingwane yomkhiqizo
Ukusetshenziswa Okuhlosiwe:
Ikhithi Yokuhlola Esheshayo Ye-Monkeypox Virus Antigen isetshenziselwa ukuthola i-antigen ye-Monkeypox kumasampula e-exudate noma ama-scab esilonda somuntu. Yenzelwe ukusetshenziswa kokuxilongwa ngaphakathi kwe-vitro kuphela.
Izimiso Zokuhlola:
Uma isampula icutshungulwa futhi ingezwa emthonjeni wesampula, ama-antigen egciwane le-monkeypox kusampula asebenzisana nama-conjugate e-antibody e-antibody color particle complexes abhalwe nge-conjugate. Ama-complexes ahamba ku-membrane ye-nitrocellulose ngesenzo se-capillary kuze kube umugqa wokuhlola, lapho abanjwa khona ama-antibodies egciwane le-monoclonal anti-monkeypox virus. Umugqa wokuhlola onombala uyabonakala efasiteleni lemiphumela uma ama-antigen egciwane le-monkeypox ekhona kusampula futhi ukuqina kuncike enanini le-antigen yegciwane le-monkeypox. Uma ama-antigen egciwane le-monkeypox kusampula engekho noma engaphansi komkhawulo wokutholwa, akukho bhande elinombala elibonakalayo kumugqa wokuhlola wedivayisi. Lokhu kubonisa umphumela ongemuhle. Umugqa wokuhlola noma umugqa wokulawula awubonakali efasiteleni lemiphumela ngaphambi kokusebenzisa isampula. Umugqa wokulawula obonakalayo uyadingeka ukukhombisa ukuthi umphumela uyasebenza.
Okuqukethwe Okuyinhloko
Izingxenye ezinikeziwe zibhalwe kuthebula.
| I-REF yengxenye | B031C-01-01 | B031C-01-25 | B031C-01-25 |
| Ikhasethi yokuhlola | Ukuhlolwa okungu-1 | Ukuhlolwa okungu-5 | Ukuhlolwa okungu-25 |
| Isixazululo sokukhipha isampula | Ibhodlela eli-1 | Ibhodlela eli-5 | Ibhodlela lama-25 |
| Indwangu yokulahla | Isiqephu esisodwa | izingcezu ezi-5 | izingcezu ezingu-25 |
| Imiyalelo yokusebenzisa | Isiqephu esisodwa | Isiqephu esisodwa | Isiqephu esisodwa |
| Isitifiketi sokuhambisana | Isiqephu esisodwa | Isiqephu esisodwa | Isiqephu esisodwa |
Ukugeleza Kokusebenza
Funda imiyalelo kahle ngaphambi kokuhlola bese uletha ikhasethi kanye nesampula ekushiseni kwegumbi. Uma usukulungele ukuhlola, vula isikhwama esisesikhaleni bese ususa idivayisi. Beka idivayisi yokuhlola endaweni ehlanzekile, eyisicaba.
Ikhithi Yokuhlola Okusheshayo Ye-Monkeypox Virus Antigen ingenziwa kumasampula e-lesion exudate noma amasampula e-scab
NgobaAmasampula e-lesion exudate noma ama-scab:
1. Sula amasampula e-exudate noma e-scab yesilonda nge-swab.
2. Faka i-swab epayipini lesisombululo sokukhipha isampula bese uphonsa i-swab phezulu naphansi oketshezini okungenani imizuzwana eyi-15, bese ubamba i-swab phansi kwepayipi bese uyijikelezisa izikhathi ezi-5.
3. Susa i-swab ngenkathi ucindezela izinhlangothi zethubhu ukuze ukhiphe uketshezi ku-swab. Cindezela isivalo se-nozzle ngokuqinile ethubhini lesisombululo eliqukethe isampula ecutshunguliwe. Hlanganisa kahle ngokuzungeza noma ngokupheqa phansi kwethubhu.
4. Hlanganisa isampula ngokuphendula kancane ithubhu libheke phansi, cindezela ithubhu ukuze wengeze amaconsi amathathu (cishe ama-100 μL) emthonjeni wesampula wekhasethi yokuhlola, bese uqala ukubala.
5. Funda umphumela ngokubuka ngemuva kwemizuzu eyi-15-20. Umphumela awusebenzi ngemuva kwemizuzu engama-20.
Ukuhumusha imiphumela
Ulwazi lwe-oda
| Igama Lomkhiqizo | Ikati. Cha | Usayizi | Isibonelo | Impilo Yeshelufu | Izinga lokushisa le-Trans. & Sto. |
| Ikhithi Yokuhlola Okusheshayo Ye-Monkeypox Virus Antigen | B031C-01 | Isivivinyo/ikhithi eli-1 | amasampula e-lesion exudate noma ama-scab | Izinyanga ezingu-24 | 2-30℃ |
| B031C-05 | 5 ukuhlolwa/ikhithi | ||||
| B031C-25 | 25 ukuhlolwa/ikhithi |
