Ikhithi Yokuhlola Esheshayo ye-S. Typhi / Paratyphi Combo Antigen (I-Immunochromatographic Assay) Isithombe Esifakiwe

Ikhithi Yokuhlola Okusheshayo Ye-S. Typhi / Paratyphi Combo Antigen (I-Immunochromatographic Assay)

Incazelo emfushane:

Isibonelo	Indle	Ifomethi	Ikhasethi
Ukuzwela	I-Typhi (93.10%) i-Paratyphi (93.41%)	Ukucaciswa	I-Typhi (92.53%) i-Paratyphi (94.59%)
Izinga lokushisa le-Trans. & Sto.	2-30℃ / 36-86℉	Isikhathi Sokuhlolwa	imizuzu eyi-10-20
Imininingwane	Isivivinyo/Ikhithi eli-1; Izivivinyo/Ikhithi ezi-5; Izivivinyo/Ikhithi ezingama-25

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Imininingwane yomkhiqizo

Ukusetshenziswa Okuhlosiwe:

I-S. Typhi/Paratyphi Combo Antigen Rapid Test Kit (I-Immunochromatographic Assay) iyisivivinyo sokugeleza okusheshayo kwe-lateral esiqhutshwa ngaphakathi kwe-vitro, esaziwa nangokuthi i-lateral flow immunochromatographic assay, esenzelwe ukuthola ama-antigen e-S. Typhi kanye ne-Paratyphi kumasampula endle avela ezigulini. Imiphumela evela ku-S. Typhi/Paratyphi Combo Antigen Rapid Test Kit kufanele ihunyushwe kanye nokuhlolwa kwesiguli kanye nezinye izindlela zokuxilonga.

Izimiso Zokuhlola:

I-S. Typhi/Paratyphi Combo Antigen Rapid Test Kit (I-Immunochromatographic Assay) iyi-immunoassay ye-chromatographic flow lateral flow. Inemigqa emithathu engaphambi kokumbozwa, umugqa we-“T1” S. Typhi Test, umugqa we-“T2” Paratyphi Test kanye nomugqa we-“C” Control on the nitrocellulose membrane. Ama-antibody e-monoclonal anti-S. Typhi kanye ne-anti-Paratyphi ambozwe endaweni yomugqa wokuhlola kanti ama-antibody e-Goat anti-chicken IgY ambozwe endaweni yokulawula. Lapho isampula icutshungulwa futhi ingezwa emthonjeni wesampula, ama-antigen e-S. Typhi/Paratyphi kusampula asebenzisana nama-conjugate e-S. Typhi/Paratyphi Antibody-labeled conjugate forming antigen-antibody color particle complexes. Ama-complex athuthela ku-membrane ye-nitrocellulose ngesenzo se-capillary kuze kube yilapho efika emgqeni wokuhlola, lapho abanjwa khona ama-antibody e-monoclonal anti-S. Typhi/Paratyphi. Umugqa we-T1 onombala uyabonakala efasiteleni lemiphumela uma ama-antigen e-S. Typhi ekhona esampula futhi ukuqina kuncike enanini le-antigen ye-S. Typhi. Umugqa we-T2 onombala uyabonakala efasiteleni lemiphumela uma ama-antigen e-Paratyphi ekhona esampula futhi ukuqina kuncike enanini le-antigen ye-Paratyphi. Uma ama-antigen e-S.Typhi/Paratyphi esampula engekho noma engaphansi komkhawulo wokutholwa, akukho bhande elinombala elibonakalayo emugqeni wokuhlola (T1 kanye ne-T2) wedivayisi. Lokhu kubonisa umphumela omubi. Umugqa wokuhlola noma umugqa wokulawula awubonakali efasiteleni lemiphumela ngaphambi kokusebenzisa isampula. Umugqa wokulawula obonakalayo uyadingeka ukukhombisa ukuthi umphumela uyasebenza

Okuqukethwe Okuyinhloko

Izingxenye ezinikeziwe zibhalwe kuthebula.

I-REF yengxenye I-REF	B033C-01	B033C-05	B033C-25
Ikhasethi Yokuhlola	Ukuhlolwa okungu-1	Ukuhlolwa okungu-5	Ukuhlolwa okungu-25
I-Buffer	Ibhodlela eli-1	Ibhodlela eli-5	Amabhodlela angu-25/2
Isikhwama Sokuthutha Sezibonelo	Isiqephu esisodwa	5 ama-pcs	25 ama-pcs
Imiyalelo Yokusebenzisa	Isiqephu esisodwa	5 ama-pcs	25 ama-pcs
Isitifiketi Sokuhambisana	Isiqephu esisodwa	Isiqephu esisodwa	Isiqephu esisodwa

Ukugeleza Kokusebenza

Isinyathelo 1: IsampulaUkulungiselela

1. Qoqa amasampula endle ezitsheni ezihlanzekile, ezingavuzi.
2. Ukuthuthwa Nokugcinwa Kwezinto Ezifanayo: Izinto ezifanayo zingagcinwa ekamelweni lokushisa amahora angu-8 noma zifakwe efrijini ku-36°F kuya ku-46°F (2°C kuya ku-8°C) amahora afinyelela kwangu-96.
3. Izibonelo zendle ezigcinwe ziqandisiwe zingancibilikiswa izikhathi ezifika kwezimbili ku--10°C noma ngaphansi. Uma usebenzisa izibonelo eziqandisiwe, ncibilikiswa ekushiseni kwegumbi. Ungavumeli izibonelo zendle zihlale engxubeni yokuxuba isikhathi esingaphezu kwamahora ama-2.

Isinyathelo 2: Ukuhlola

1. Sicela ufunde imiyalelo ngokucophelela ngaphambi kokuhlola. Ngaphambi kokuhlola, vumela amakhasethi okuhlola, isixazululo sesampula kanye namasampula ukuthi alingane nokushisa kwegumbi (15-30℃ noma 59-86 degrees Fahrenheit).

2. Susa ikhasethi yokuhlola esikhwameni se-foil bese uyibeka endaweni eyisicaba.

3. Khulula ibhodlela lesampula, sebenzisa induku yokufaka enamathiselwe enamathiselwe esivalweni ukuze udlulisele ingxenye encane yesampula yendle (ububanzi obungu-3-5 mm; cishe u-30-50 mg) ebhodleleni lesampula eliqukethe i-buffer yokulungiselela isampula.

4. Buyisela induku ebhodleleni bese uqinisa kahle. Hlanganisa isampula yendle ne-buffer kahle ngokushukumisa ibhodlela izikhathi eziningana bese ushiya ithubhu lodwa imizuzu emi-2.

5. Khulula isihloko sebhodlela lesampula bese ubamba ibhodlela liqonde phezulu phezu komthombo wesampula weCassette, ulethe amaconsi amathathu (100 -120μL) esampula yendle exutshwe emanzini emthonjeni wesampula.

Isinyathelo 3: Ukufunda

Funda imiphumela ngemizuzu eyi-15-20. Isikhathi sokuchaza imiphumela asidluli imizuzu engama-20

Ukuhumusha imiphumela

1. Umphumela Omuhle ka-S. Typhi

Amabhande anombala avela kokubili emgqeni wokuhlola (T1) kanye nomugqa wokulawula (C). Kubonisa umphumela omuhle wama-antigen e-S. Typhi kusampula.

2. Umphumela Omuhle we-Paratyphi

Amabhande anombala avela kokubili emgqeni wokuhlola (T2) kanye nomugqa wokulawula (C). Lokhu kubonisa umphumela omuhle wama-antigen e-Paratyphi kusampula.

3. Umphumela Omuhle ka-S. Typhi no-Paratyphi

Amabhande anombala avela kokubili emgqeni wokuhlola (T1), emgqeni wokuhlola (T2) kanye nomugqa wokulawula (C). Kubonisa umphumela omuhle wama-antigen e-S. Typhi kanye ne-Paratyphi kusampula.

4. Umphumela Omubi

Ibhendi enemibala ivela emgqeni wokulawula (C) kuphela. Ibonisa ukuthi ukuhlushwa kwama-antigen e-S. Typhi noma e-Paratyphi akukho noma akukho ngaphansi komkhawulo wokutholwa kokuhlolwa.

5. Umphumela Ongavumelekile

Akukho bhande elinombala elibonakalayo elivela emgqeni wokulawula ngemva kokwenza isivivinyo. Iziqondiso kungenzeka ukuthi azilandelwanga kahle noma isivivinyo kungenzeka ukuthi sonakele. Kunconywa ukuthi isampula ihlolwe kabusha

Ulwazi lwe-oda

Igama Lomkhiqizo	Ikati. Cha	Usayizi	Isibonelo	Impilo Yeshelufu	Izinga lokushisa le-Trans. & Sto.
Ikhithi Yokuhlola Okusheshayo Ye-S. Typhi/Paratyphi Combo Antigen (I-Immunochromatographic Assay)	B033C-01	Isivivinyo/ikhithi eli-1	Indle	Izinyanga ezingu-24	2-30℃
	B033C-05	Ukuhlolwa okungu-5/ikhithi
	B033C-25	Ukuhlolwa okungu-25/ikhithi