I-Recombinant Monkeypox Virus A35R Protein, C-Ithegi yakhe

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Umthombo	I-Monkeypox Virus(strain Zaire-96-I-16)
Expression Host	HEK 293 Amaseli
Maka	C-Ithegi yakhe
Isicelo	Ilungele ukusetshenziswa kuma-immunoassays. Ilabhorethri ngayinye kufanele inqume i-titer yokusebenza efanele ukuze isetshenziswe ezinhlelweni zayo ikakhulukazi.
Ulwazi oluvamile	I-recombinant monkeypox virus A35R protein ikhiqizwa i-Mammalian expression system kanye isakhi sofuzo esiqondiwe esifaka ikhodi i-Arg58-Thr181 ivezwa nge-His-tag ku-C-terminus.

Ubumsulwa	>95% njengoba kunqunywa i-SDS-PAGE.
I-Molecular Mass	I-recombinant monkeypox virus A35R protein ehlanganisa ama-amino acid ayi-139 futhi inesisindo samangqamuzana esibaliwe esingu-15.3 kDa.Iphrotheni ifuduka njenge-15-26 kDa ngaphansi kokunciphisa i-SDS-PAGE ngenxa ye-glycosylation.
Isilondolozi somkhiqizo	20 mM Tris, 10 mM NaCl, pH 8.0.
Isitoreji	Igcine ngaphansi kwezimo eziyinyumba ku -20℃ ukuya ku -80 ℃ uma yamukelwa. Ncoma ukuthi i-aliquot iphrotheni ibe ngamanani amancane ukuze uthole isitoreji esiphezulu.