Ikhithi Yokuhlola I-Leishmania IgG/IgM (I-Immunochromatographic Assay)

Incazelo emfushane:

Isifanekiso	ISerum/Plasma/Igazi Eliphelele	Ifomethi	Ikhasethi
Ukuzwela	98.83%	Ukucaciswa	98.02%
Trans.& Sto.Temp.	2-30℃ / 36-86℉	Isikhathi Sokuhlola	5-10 amaminithi
Ukucaciswa	1 Test/Kit 5 Tests/Kit 25 Tests/Kit

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane yomkhiqizo

Ukusetshenziswa Okuhlosiwe
Lo mkhiqizo ulungele ukuhlolwa komtholampilo kwekhwalithi ye-serum/plasma/amasampula egazi lonke ukuze kutholwe amasosha omzimba ngokumelene ne-Leishmania.Kuyisivivinyo esilula, esisheshayo nesingenazinsimbi sokuxilongwa kwe-kala-azar ebangelwa yi-Leishmania.

Isimiso Sokuhlola
Lo mkhiqizo i-lateral flow chromatographic immunoassay.Ikhasethi lokuhlola liqukethe: 1) i-burgundy colored conjugate pad equkethe i-recombinant rK39 antigen ehlanganiswe negolide le-colloid (ama-conjugates e-Leishmania) kanye nama-conjugate kanogwaja we-IgG-gold;2) umugqa we-nitrocellulose membrane oqukethe amabhendi amabili okuhlola (amabhendi M no-G) kanye nebhendi yokulawula (ibhendi C).

Okuqukethwe Okuyinhloko

Izingxenye ezinikeziwe zibalwe kuthebula.

Izinto zokwakha / enikeziwe	Ubuningi (Isivivinyo esi-1/Ikhithi)	Inani(5Test/Kit)	Inani(25Test/Kit)
Ikhithi Yokuhlola	1 ukuhlola	5 izivivinyo	25 izivivinyo
Ibhafa	1 ibhodlela	5 amabhodlela	25/2 amabhodlela
I-Dropper	1 isiqephu	5 ama-pcs	25 ama-pcs
Isikhwama Sokuhamba Sesampula	1 isiqephu	5 ama-pcs	25 ama-pcs
I-Lancet elahlayo	1 isiqephu	5 ama-pcs	25 ama-pcs
Imiyalo Yokusetshenziswa	1 isiqephu	1 isiqephu	1 isiqephu
Isitifiketi Sokuhambisana	1 isiqephu	1 isiqephu	1 isiqephu

Ukugeleza Kokusebenza

Isinyathelo 1: Isampula

Qoqa iSerum yomuntu/Plasma/Igazi Eliphelele ngendlela efanele.

Isinyathelo sesi-2: Ukuhlola

Sicela ufunde imiyalelo ngokucophelela ngaphambi kokuhlolwa.Ngaphambi kokuhlola, vumela ikhithi yokuhlola, isisombululo sesampula kanye nesampula ukuthi kulingane nezinga lokushisa (15-30℃noma 59-86 degrees Fahrenheit).
1.Khipha ishubhu lokukhipha ekhithi kanye nebhokisi lokuhlola esikhwameni sefilimu ngokuklebhula inotshi.Zibeke endizeni enezingqimba.
2.Vula ikhadi lokuhlola isikhwama se-aluminium foil.Susa ikhadi lokuhlola bese ulibeka livundlile etafuleni.
3.Sebenzisa ipayipi elahlwayo, dlulisela iconsi elilodwa (cishe 20μL) igazi lomunwe/noma 4μL serum / noma 4μL plasma/ noma 4μL lonke igazi emthonjeni wesampula kukhasethi lokuhlola.
4.Vula ishubhu lebhafa.Faka amaconsi ama-3 (cishe ama-80 μL) e-assay diluent ku-assay diluent eyindilinga kahle.Qala ukubala.

Isinyathelo sesi-3: Ukufunda

Funda umphumela ngemizuzu emi-5-10.Imiphumela ngemuva kwemizuzu eyi-10 ayivumelekile.

Ukuhunyushwa komphumela

Umphumela ongemuhle
Uma kuphela ulayini wokulawula ikhwalithi uvela futhi imigqa yokuthola u-G no-M ingabonisi

Umphumela Omuhle
1.Kokubili ulayini wokulawula ikhwalithi C kanye nolayini wokuthola u-M uyavela= i-antibody ye-Leishmania IgM itholiwe, futhi umphumela uba positive ku-antibody ye-IgM.
2.Kokubili ulayini wokulawula ikhwalithi C kanye nolayini wokuhlonza ongu-G uyavela= i-antibody ye-Leishmania IgG itholakele futhi umphumela uba omuhle ku-antibody ye-IgG.
3.Kokubili umugqa wokulawula ikhwalithi C kanye nemigqa yokuhlonza u-G no-M uyavela=amasosha omzimba e-Leishmania IgG kanye ne-IgM.ziyatholwa, futhi umphumela uba muhle kuwo womabili amasosha omzimba e-IgG kanye ne-IgM.

Umphumela Ongavumelekile
Ulayini wokulawula ikhwalithi C awukwazi ukubonwa, imiphumela izoba engavumelekile kungakhathaliseki ukuthi umugqa wokuhlola uyabonisa, futhi ukuhlola kufanele kuphindwe.

Oda Ulwazi

Igama Lomkhiqizo	Ikati.Cha	Usayizi	Isifanekiso	I-Shelf Life	Trans.& Sto.Temp.
Ikhithi Yokuhlola I-Leishmania IgG/IgM (I-Immunochromatographic Assay)	B020C-01	1 test/kit	ISerum/Plasma/Igazi Eliphelele	Izinyanga ezingu-18	2-30℃ / 36-86℉
	B020C-05	Izivivinyo ezi-5/ikhithi
	B020C-25	Izivivinyo ezingama-25/ikhithi