Ikhithi Yokuhlola Esheshayo Ye-Cardiac Troponin I (Lateral Chromatography)
Imininingwane yomkhiqizo
Ukusetshenziswa okuhlosiwe:
I-Cardiac Troponin I Rapid Test Kit isebenzisa i-colloidal gold immunochromatography ukuze kutholwe i-Troponin I (cTnI) yenhliziyo ku-serum, i-plasma noma isampula yegazi lonke ngokwekhwalithi noma kancane kancane ngekhadi elijwayelekile le-colorimetric.Lokhu kuhlolwa kusetshenziselwa ukuxilongwa kokulimala kwe-myocardial njenge-Acute Myocardial Infarction, I-Angina Engaqinile, I-Acute Myocarditis kanye ne-Acute Coronary Syndrome.
Izimiso Zokuhlola:
Ikhithi Yokuhlola Esheshayo Ye-Cardiac Troponin I (Lateral Chromatography) iyikhwalithi noma i-semi-quantitative, i-membrane based immunoassay ukuze kutholwe inhliziyo ye-Troponin I(cTnI) egazini lonke, i-serum noma i-plasma.Kule nqubo yokuhlola, i-reagent yokuthwebula ayinyakazi esifundeni somugqa wokuhlola wokuhlola.Ngemva kokuba isifanekiso sengezwe endaweni yesampula yekhasethi, sibhekana nezinhlayiya ezimboze i-anti-cTnI ekuhlolweni.Le ngxube ithutha ngokwechromatographic ngobude bokuhlolwa futhi ihlanganyele ne-immobilized capture reagent.Ifomethi yokuhlola ingathola i-Troponin I(cTnI) yenhliziyo kuma-specimens.Uma isifanekiso siqukethe i-Troponin I(cTnI yenhliziyo yenhliziyo), kuzovela umugqa onemibala endaweni yomugqa wokuhlola futhi ukushuba kombala komugqa wokuhlola kukhuphuka ngokulingana nokugxilisana kwe-cTnI, okubonisa umphumela omuhle.Uma isifanekiso singaqukethe i-Troponin I(cTnI yenhliziyo), umugqa onombala ngeke uvele kulesi sifunda, okubonisa umphumela ongemuhle.Ukuze usebenze njengesilawuli senqubo, umugqa onemibala uzohlala uvela endaweni yokulawula, okubonisa ukuthi umthamo ofanele we-specimen ungeziwe futhi i-membrane wicking yenzekile.
Okuqukethwe Okuyinhloko
Izingxenye ezinikeziwe zibalwe kuthebula.
| Ingxenye REF REF | B032C-01 | B032C-25 |
| Hlola ikhasethi | 1 ukuhlola | 25 izivivinyo |
| I-diluent yesampula | 1 ibhodlela | 1 ibhodlela |
| I-Dropper | 1 isiqephu | 25 ama-pcs |
| Ikhadi elijwayelekile le-colorimetric | 1 isiqephu | 1 isiqephu |
| Isitifiketi Sokuhambisana | 1 isiqephu | 1 isiqephu |
Ukugeleza Kokusebenza
Isinyathelo 1: Ukulungiselela Isampula
1. Ikhithi yokuhlola ingenziwa kusetshenziswa igazi lonke, i-serum noma i-plasma.Phakamisa ukukhetha i-serum noma i-plasma njengesampula yokuhlola.Uma ukhetha igazi eliphelele njengesampula lokuhlola, kufanele lisetshenziswe kanye nesampula yegazi elixutshiwe.
2. Hlola isampula ekhadini lokuhlola ngokushesha.Uma ukuhlolwa kungeke kuqedwe ngokushesha, isampula le-serum ne-plasma kufanele ligcinwe kuze kufike ezinsukwini ezingu-7 ku-2 ~ 8 ℃ noma ligcinwe ku--20 ℃ izinyanga ezingu-6 (isampula lonke legazi kufanele ligcinwe kuze kube yizinsuku ezingu-3 ku-2 ~ 8℃ ) ize ihlolwe.
3. Amasampula kufanele abuyiselwe ekamelweni lokushisa ngaphambi kokuhlolwa.Amasampula aqandisiwe adingeka ukuthi ancibilikiswe ngokuphelele futhi axutshwe kahle ngaphambi kokuhlolwa, ukugwema ukuqanda nokuncibilika ngokuphindaphindiwe.
4. Gwema ukushisa amasampula, okungabangela i-hemolysis kanye ne-protein denaturation.Kunconywa ukugwema ukusebenzisa isampula ye-hemolyzed kakhulu.Uma isampula ibonakala ine-hemolyzed kakhulu, kufanele kutholwe elinye isampula futhi lihlolwe.
Isinyathelo sesi-2: Ukuhlola
1. Sicela ufunde imanuwali ngokucophelela ngaphambi kokuhlolwa, buyisela isampula, ikhadi lokuhlola kanye nesampula yegazi elincibilikisiwe kumazinga okushisa ekamelo bese ufaka inombolo yekhadi.Phakamisa ukuvula isikhwama se-foil ngemva kokululama ezingeni lokushisa legumbi nokusebenzisa ikhadi lokuhlola ngokushesha.
2. Beka ikhadi lokuhlola etafuleni elihlanzekile, elibekwe ngokuvundlile.
Ngeserum noma isampula sePlasma:
Bamba i-dropper ngokuqondile bese udlulisela amaconsi angu-3 e-serum noma i-plasma (cishe ama-80 L, i-Pipette ingasetshenziswa esimweni esiphuthumayo) ku-specimen kahle, bese uqala isibali sikhathi.Bheka umfanekiso ngezansi.
Ngesifanekiso Segazi Eliphelele:
Bamba i-dropper ngokuqondile bese udlulisela amaconsi angu-3 egazi eliphelele (cishe ama-80 L) esibonelweni kahle, bese wengeza iconsi elingu-1 le-diluent yesampuli (cishe 40 L), bese uqala isibali sikhathi.Bheka umfanekiso ngezansi.
Isinyathelo sesi-3: Ukufunda
Emaminithini angu-10 ~ 30, thola umphumela we-semi-quantitative ngokwekhadi elijwayelekile le-colorimetric ngamehlo.
Ukutolika imiphumela
Kuvumelekile: Ukulandelana okubomvu oku-purplish okukodwa kuvela kulayini wokulawula (C).Mayelana nemiphumela evumelekile, ungathola i-semi-quantitative ngamehlo ngekhadi elijwayelekile le-colorimetric:
Ukuqina Kombala vs Ukugxila Kwereferensi
| Ukuqina Kombala | Ukugxila Kwereferensi (ng / ml) |
| - | ＜0.5 |
| + - | 0.5~1 |
| + | 1~5 |
| + + | 5~15 |
| +++ | 15-30 |
| ++++ | 30-50 |
| ++++ | ＞50 |
Akuvumelekile: Akukho ukugqama okubomvu oku-purplish okuvela emugqeni wokulawula(C).Lokhu kusho ukuthi ukusebenza okuthile kufanele kube okungalungile noma ikhadi lokuhlola selivele lingavumelekile.Kulesi simo sicela ufunde imanuwali ngokucophelela futhi, bese uzama futhi ngekhasethi elisha lokuhlola.Uma isimo esifanayo senzeka futhi, kufanele uyeke ukusebenzisa le qoqo lemikhiqizo ngokushesha futhi uxhumane nomphakeli wakho.
Oda Ulwazi
| Igama Lomkhiqizo | Ikati.Cha | Usayizi | Isifanekiso | I-Shelf Life | Trans.& Sto.Temp. |
| Ikhithi Yokuhlola Esheshayo Ye-Cardiac Troponin I (Lateral Chromatography) | B032C-01 | 1 isivivinyo/ikhithi | I-S/P/WB | Izinyanga ezingama-24 | 2-30 ℃ |
| B032C-25 | Izivivinyo ezingama-25/ikhithi |