I-Yeast Cell Protein Expression
I-yeast expression system iyindlela esetshenziswa kakhulu yokuvezwa kwamaprotheni e-eukaryotic, ngenxa yobulula bayo ekulimeni, ukufinyeleleka, kanye nokusebenza kalula.Phakathi kwezinhlobo ezihlukahlukene zemvubelo, i-Pichia pastoris ingumsingathi wenkulumo ethandwa kakhulu, njengoba isiza kokubili ukubonakaliswa kwe-intracellular kanye ne-extracellular protein.Uhlelo futhi luvumela ukuguqulwa kwangemva kokuhumusha, okufana ne-phosphorylation kanye ne-glycosylation, okuholela kusistimu ye-eukaryotic expression enezinzuzo eziningi.
| Izinto Zesevisi | Isikhathi Sokuhola (BD) |
| Ukwenza ngcono kwe-Codon, ukuhlanganiswa kofuzo kanye ne-subcloning | 5-10 |
| Ukuhlolwa kwe-clone okuhle | 10-15 |
| Inkulumo yesikali esincane | |
| Inkulumo nokuhlanzwa kwesilinganiso esikhulu (200ML), okulethwayo kufaka phakathi amaprotheni ahlanziwe kanye nombiko wokuhlola |
Uma isakhi sofuzo sihlanganiswa ku-Bioantibody, i-plasmid eyakhiwe izofakwa kokulethwayo.