I-Recombinant Viral Dengue Virus 2 NS1 Protein, C-Ithegi yakhe

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Umthombo	Iseli yezinso zombungu womuntu
Expression Host	Huma
Maka	C-Ithegi yakhe
Isicelo	Ilungele ukusetshenziswa kuma-immunoassays. Ilabhorethri ngayinye kufanele inqume i-titer yokusebenza efanele ukuze isetshenziswe ezinhlelweni zayo ikakhulukazi.
Ulwazi oluvamile	I-recombinant viral dengue virus 2 NS1 protein ikhiqizwa amaseli omuntu angama-293 (HEK293) futhi isakhi sofuzo esihloselwe ukubhala ngekhodi i-Asp776 - Ala1127 ivezwa ngo-6-Tag Yakhe ku-C-terminus.

Ubumsulwa	>95% njengoba kunqunywa i-SDS-PAGE.
Amangqamuzana IMisa	Iphrotheni ine-MW ebaliwe yama-44 kDa.Iphrotheni ifuduka njenge-44-60 kDa ngaphansi kwesimo sokunciphisa (R) (SDS-PAGE) ngenxa ye-glycosylation ehlukile.
Isilondolozi somkhiqizo	10 mM PB, 150 mM NaCl, pH 8.0.
Isitoreji	Igcine ngaphansi kwezimo eziyinyumba ku -20℃ ukuya ku -80 ℃ uma yamukelwa. Ncoma ukuthi i-aliquot iphrotheni ibe ngamanani amancane ukuze uthole isitoreji esiphezulu.