Recombinant Rotavirus A Intermediate Capsid Protein VP6, N-Ithegi Yakhe

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Umthombo	Umuntu
Expression Host	I-Rotavirus A
Maka	N-Ithegi yakhe
Isicelo	Ilungele ukusetshenziswa kuma-immunoassays. Ilabhorethri ngayinye kufanele inqume i-titer yokusebenza efanele ukuze isetshenziswe ezinhlelweni zayo ikakhulukazi.
Ulwazi oluvamile	I-Recombinant rotavirus Iphrotheni ye-capsid ephakathi nendawo i-VP6 ikhiqizwa isistimu yokusho ye-E.coli kanye nombhalo wekhodi wofuzo i-Met1-Lys397 uvezwa nge-His-tag ku-N-terminus.

Ubumsulwa	>90% njengoba kunqunywa i-SDS-PAGE.
I-Molecular Mass	I-recombinant rotavirus A Intermediate Capsid Protein VP6 ehlanganisa ama-amino acid angu-412 futhi inesisindo samangqamuzana esibaliwe esingaba ngu-46.6 kDa.
Isilondolozi somkhiqizo	10 mM PB, 6% sucrose, pH 8.0.
Isitoreji	Igcine ngaphansi kwezimo eziyinyumba ku -20℃ ukuya ku -80 ℃ uma yamukelwa. Ncoma ukuthi i-aliquot iphrotheni ibe ngamanani amancane ukuze uthole isitoreji esiphezulu.