I-Recombinant Helicobacter pylori CagA protein, N-Ithegi Yakhe
Ulwazi oluvamile
| Umthombo | Umuntu |
| Expression Host | E.coli |
| Maka | N-Ithegi yakhe |
| Isicelo | Ifanele ukusetshenziswa kuma-immunoassays. Ilabhorethri ngayinye kufanele inqume i-titer ephezulu yokusebenza ukuze isetshenziswe ezinhlelweni zayo ezithile. |
| Ulwazi oluvamile | I-Recombinant Helicobacter pylori CagA ikhiqizwa isistimu yokusho ye-E.coli kanye ne-targetgene encoding Met1-Phe294 ivezwa nge-His-tag ku-N-terminus. |
Izakhiwo
| Ubumsulwa | >90% njengoba kunqunywa i-SDS-PAGE.
|
| Amangqamuzana IMisa | Iprotheni ye-helicobacter pylori CagA iqukethe ama-amino acid angama-308 futhi inesisindo samangqamuzana esibaliwe esingaba ngu-35.2 kDa. |
| Isilondolozi somkhiqizo | 10 mM PB, pH 8.0. |
| Isitoreji | Igcine ngaphansi kwezimo eziyinyumba ku -20℃ ukuya ku -80 ℃ uma yamukelwa. Ncoma ukuthi i-aliquot iphrotheni ibe ngamanani amancane ukuze uthole isitoreji esiphezulu. |
Oda Ulwazi
| Igama Lomkhiqizo | Ikati.Cha | Ubuningi |
| I-Recombinant Helicobacter pylori CagA protein, N-Ithegi Yakhe
| AG0119 | Ngokwezifiso |
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