Isevisi ye-Monoclonal Antibody
I-Monoclonal antibody, ekhiqizwa i-B cell clone eyodwa, inokulingana okuphezulu okuqondise i-epitope ye-antigen ethile.I-Monoclonal antibody inezinzuzo eziningi, okuhlanganisa ukuhlanzeka okuphezulu, ukuzwela, nokucacisa.Ama-Hybridoma adalwa ngokuhlanganiswa kwama-lymphocyte B akhiqiza amasosha omzimba athile, anamaseli e-myeloma aphila isikhathi eside.I-Bioantibody isebenzisa ubuchwepheshe bokuhlanganisa obusebenza kahle izikhathi ezingama-20 kunezindlela ezijwayelekile zokuhlanganisa.Ukwengeza, isebenzisa ubuchwepheshe bokuhlola i-protein microarray ukukhomba amasosha omzimba e-monoclonal abonisa ukucaciswa okuphezulu, ukuhambisana, nokusebenza ngempumelelo ngokumelene nama-epitopes athile.
| Izinto Zesevisi | Okuqukethwe Kokuhlola | Isikhathi Sokuhola (Iviki) |
| Ukulungiswa kwe-antigen | 1. Ikhasimende lihlinzeka nge-antigen2. I-bioantibody ilungisa i-antigen | / |
| Ukugonywa kwegundane | Ukugonywa kwegundane le-BALB/c, ukuqoqwa kweserum nokuhlaziywa kwe-ELISA | 4 |
| Ukuhlanganiswa kwamaseli nokuhlolwa | Ukuhlanganiswa kwama-splenocyte egundane namaseli e-myeloma, ukuhlolwa kwe-HAT | 2 |
| Ukusungulwa kolayini wamaseli okuzinzile | Ukwenziwa kwe-subcloning yama-clones amahle ahloliwe | 3 |
| Ukuhlonza isotype ye-antibody | Ukuhlonza ama-subtypes womugqa weseli | 1 |
| I-incubation encane | Ukufakwa kwe-serum-free incubation | 2 |
| I-incubation enkulu kanye nokuhlanza | 200mL incubation engena serum kanye nokuhlanza | 1 |
