I-Gene Synthesis
Ngosizo lwenkundla yokuhlanganisa izakhi zofuzo esezingeni eliphezulu kanye ne-R&D enolwazi nethimba labaphathi bephrojekthi, i-Bioantibody inekhono lokuhlanganisa izakhi zofuzo zanoma ibuphi ubude nokulandelana kwazo ngokunemba okukhulu.Ngaphezu kwalokho, i-Bioantibody inweba izinsiza ezitholakalayo zokuthuthukisa i-codon kumakhasimende ayo futhi inikeza intaphane yezinketho ze-vector ezivela kumtapo wezincwadi wayo obanzi ohlanganisa ama-vector angaphezu kwekhulu.Kubalulekile ukuqaphela ukuthi ngaphandle kokuthi kucaciswe ngokucacile, i-Bioantibody isebenzisa i-pUC57 ye-plasmid vector enekhophi ephezulu ngezinjongo zokuhlanganisa izakhi zofuzo.Ngaphandle kwalezi zinsizakalo, i-Bioantibody futhi ihlinzeka ngezinsizakalo zamahhala ze-vector subcloning ezihlangabezana nezidingo ezithile zamakhasimende ayo.I-Bioantibody iphinde inikeze ngezinsizakalo ezihlanganisa isakhi sofuzo esiqondiswe kuma-vector anikezwa ngamakhasimende, kanye nesevisi yokugcina i-vector.
Ubude beGene (bp) | Isikhathi Sokuhola (BD) |
<500 | 5 |
500~3,000 | 5~10 |
3,001~5,000 | 10~15 |
5,001~8,000 | 15-20 |
>8,000 | 20~25 |