Ikhithi yokuhlola ye-SARS-CoV-2 Neutralizing antibody (Lateral chromatography)

Incazelo emfushane:

Isifanekiso	I-S/P/WB	Ifomethi	Ikhasethi
Ukuzwela	97.73%	Ukucaciswa	98.71%
Trans.& Sto.Temp.	2-30℃ / 36-86℉	Isikhathi Sokuhlola	10 imiz
Ukucaciswa	Isivivinyo esi-1/Ikhithi;25 Izivivinyo/Kit

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Ukusetshenziswa Okuhlosiwe

Ikhithi yokuhlola ye-SARS-CoV-2 Neutralizing antibody (i-Lateral chromatography) ifanele ukutholwa kwekhwalithi ye-in vitro ngokushesha kwe-SARS-CoV-2 amasosha omzimba angathathi hlangothi ku-uman serum, i-plasma, noma amasampula egazi lonke (i-capillary noma i-venous).Ikhithi ihloselwe ukusiza ukuhlola impendulo eguquguqukayo yamasosha omzimba ku-SARS-CoV-2.Ukusetshenziswa kwe-in vitro diagnostic kuphela.Okokusetshenziswa kochwepheshe kuphela.

Isimiso Sokuhlola

I-SARS-CoV-2 Neutralizing Antibody Test Kit (Lateral chromatography) iyi-immunoassay esekelwe kulwelwesi efanele yokuthola amasosha omzimba akwa-SARS-CoV-2 RBD ku-serum, iplasma kanye negazi lonke.Isampula iphonswa emthonjeni wesampula bese isampula ye-dilution buffer iyengezwa ngokulandelayo.Amasosha omzimba akwa-SARS-CoV-2 RBD kusampula ahlangana namaprotheni e-RBD abhalwe yizinhlayiyana futhi akhe ama-immune complexes.Njengoba inkimbinkimbi ifuduka kulwelwesi lwe-nitrocellulose ngesenzo se-capillary, amasosha omzimba e-RBD angabanjwa elinye iphrotheni ye-RBD ehlanganiswe endaweni yokuhlola (umugqa we-T), enze umugqa wesiginali.Indawo yokulawula ikhwalithi imbozwe nge-anti-chicken IgY, futhi inkukhu ebhalwe yizinhlayiyana i-IgY iyabanjwa ukuze yakhe inkimbinkimbi kanye nokuhlanganisa emgqeni u-C.Uma ulayini C ungabonakali, ubonisa ukuthi umphumela awuvumelekile, futhi kuyadingeka ukuhlola kabusha.

Okuqukethwe Okuyinhloko

Izingxenye ezinikeziwe zibalwe kuthebula.

Ingxenye/REF	B006C-01	B006C-25
Hlola Ikhasethi	1 ukuhlola	25 izivivinyo
Iphedi Lotshwala	1 isiqephu	25 ama-pcs
I-Diluent yesampula	1 ibhodlela	25 amabhodlela
Imiyalo Yokusetshenziswa	1 isiqephu	1 isiqephu
I-Lancet elahlayo	1 isiqephu	25 ama-PC
I-Dropper	1 isiqephu	25 ama-PC
Isitifiketi Sokuhambisana	1 isiqephu	1 isiqephu

Ukugeleza Kokusebenza

Isinyathelo 1: Isampula
Qoqa iSerum yomuntu/Plasma/Igazi Eliphelele ngendlela efanele.

Isinyathelo sesi-2: Ukuhlola

1. Vula isikhwama se-aluminium foil sekhadi lokuhlola.Susa ikhadi lokuhlola bese ulibeka livundlile etafuleni.

2. Sebenzisa ipayipi elahlwayo, dlulisela i-serum engu-10µL / noma i-plasma engu-10µL/ noma engu-20µL lonke igazi emthonjeni wesampula kukhasethi lokuhlola.

3. Vula ishubhu lebhafa ngokusonta usuke phezulu.Bamba ibhodlela le-buffer liqonde phezulu kanye no-1 cm ngaphezu kwebhafa kahle.Engeza amaconsi amathathu (cishe u-100 µL) we-buffer ku-buffer kahle ekhasethini lokuhlola.

Isinyathelo sesi-3: Ukufunda
Ngemuva kwemizuzu eyi-10, funda imiphumela ngokubuka.(Qaphela: yenzaCHAfunda imiphumela ngemva kwemizuzu engu-15!)

Ividiyo

Ukuhunyushwa komphumela

Umphumela Omuhle

Uma kokubili ulayini wokulawula ikhwalithi u-C kanye nolayini we-T wokuhlonza kuvela, kusho ukuthi amasosha omzimba we-SARS-CoV-2 atholiwe, futhi umphumela uba muhle ekunciphiseni amasosha omzimba.

Umphumela Omubi

Uma kuphela ulayini we-C wokulawula ikhwalithi uvela futhi ulayini we-T wokutholwa ungabonisi umbala, kusho ukuthi amasosha omzimba a-SARS-CoV-2 awenza neutralizing awakatholwa futhi umphumela uba mubi.

Umphumela Ongavumelekile

Uma ulayini we-C wokulawula ikhwalithi ungenakubonwa, umphumela awuvumelekile kungakhathaliseki ukuthi kukhona ukuboniswa komugqa wokuthola, futhi ukuhlola kufanele kuphindwe.

Oda Ulwazi

Igama Lomkhiqizo	Ikati.Cha	Usayizi	Isifanekiso	I-Shelf Life	Trans.& Sto.Temp.
Ikhithi yokuhlola ye-SARS-CoV-2 Neutralizing antibody (Lateral chromatography)	B006C-01	1 test/kit	I-S/P/WB	Izinyanga ezingu-18	2-30℃ / 36-86℉
	B006C-25	Izivivinyo ezingama-25/ikhithi	I-S/P/WB	Izinyanga ezingu-18	2-30℃ / 36-86℉